研究方向： 光合细菌是地球上最早出现的生物之一，迄今已有30多亿年的历史，是研究光合作用的重要模式生物。地球上仍存在着大量在极端环境条件（酸、碱、热、寒、盐）下生长的光合细菌，这些微生物作为研究光合作用生理化学极限的理想模型，对于了解光合作用的起源与进化具有极其重要的意义。本实验室致力于探索极端光合微生物的光合作用及其相关的生理代谢网络相互关系等研究，运用生物化学、物理化学、结构生物学和分子生物学等学科的理论与技术，通过对关键蛋白复合物的结构与功能进行研究，揭示其中的分子机制，为进一步提高人工光电太阳能电池的捕光效率以及开发新型的生物燃料电池奠定理论基础。研究方向包括： （1）光合膜蛋白复合物的结构与功能 （2）光合作用原初反应机制 （3）光合生物极端环境适应分子机制

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  马菲

  副研究员

  fma@ibcas.ac.cn

  010-62836430

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  邹美娟

  高级工程师

  mjzou@ibcas.ac.cn

  010-62836433

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  齐晨辉

  2019级 博士

  qichenhui@126.com

  010-62836430

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  王光磊

  2020级 博士

  wangguanglei20@ucas.ac.cn

  010-62836430

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  闫一皓

  2021级 博士

  3092588326@qq.com

  010-62836433

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  王香平

  2022级 博士

  2239195929@qq.com

  010-62836433

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  王璐

  2023级 博士

  wanglu201@mails.ucas.ac.cn

  010-62836430

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  侯余勇

  2023级 博士（在职）

  hou_yy@tib.cas.cn

  010-62836433

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  许宝方

  2021级 硕士

  xubaofang21@mails.ucas.ac.cn

  010-62836430

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  岳星宇

  2021级 硕士

  yuexingyu21@mails.ucas.ac.cn

  010-62836430

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  吴雨露

  2022级 硕士

  wuyulu22@mails.ucas.ac.cn

  010-62836433

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  王温乔

  2023级 硕士

  wangwenqiao23@mails.ucas.ac.cn

  010-62836433

Yan YH, Wang GL, Yue XY, Ma F, Madigan MT, Wang-Otomo ZY, Zou MJ, Yu LJ (2024). Molecular structure and characterization of the Thermochromatium tepidum light-harvesting 1 photocomplex produced in a foreign host. Biochim. Biophys. Acta. 1865 (3): 149050.

Wang XP, Yu B, Qi CH, Wang GL, Zou M, Zhang C, Yu LJ, Ma F (2024). Energy Transfer and Exciton Relaxation in B880-B800-RC Complex through Two-Dimensional Electronic Spectroscopy. J. Phys. Chem. Lett. 15 (13): 3619-3626.

Tani K, Kanno R, Harada A, Kobayashi Y, Minamino A, Takenaka S, Nakamura N, Ji XC, Purba ER, Hall M, Yu LJ, Madigan MT, Mizoguchi A, Iwasaki K, Humbel BM, Kimura Y,  Wang-Otomo ZY (2024). High-resolution structure and biochemical properties of the LH1-RC photocomplex from the model purple sulfur bacterium, Allochromatium vinosum. Commun Biol. 7 (1): 176.

Qi CH, Wang GL, Wang FF, Wang J, Wang XP, Zou MJ, Ma F, Madigan MT, Kimura Y, Wang-Otomo ZY, Yu LJ (2024). Structural insights into the unusual core photocomplex from a triply extremophilic purple bacterium, Halorhodospira halochloris. J. Integr. Plant Biol.

Li X, Li Z, Wang F, Zhao S, Xu C, Mao Z, Duan J, Feng Y, Yang Y, Shen L, Wang G, Yang Y, Yu LJ, Sang M, Han G, Wang X, Kuang T, Shen JR, Wang W (2024). Structures and organizations of PSI-AcpPCI supercomplexes from red tidal and coral symbiotic photosynthetic dinoflagellates. Proc. Natl. Acad. Sci. U. S. A. 121 (7): e2315476121.

Li H, Nakajima Y, Nango E, Owada S, Yamada D, Hashimoto K, Luo F, Tanaka R, Akita F, Kato K, Kang J, Saitoh Y, Kishi S, Yu H, Matsubara N, Fujii H, Sugahara M, Suzuki M,   Masuda T, Kimura T, Thao TN, Yonekura S, Yu LJ, Tosha T, Tono K, Joti Y, Hatsui T,   Yabashi M, Kubo M, Iwata S, Isobe H, Yamaguchi K, Suga M, Shen JR (2024). Oxygen-evolving photosystem II structures during S1–S2–S3 transitions. Nature

Hao JF, Qi CH, Yu BY, Wang HY, Gao RY, Yamano N, Ma F, Wang P, Xin YY, Zhang CF, Yu LJ, Zhang JP (2024). Light-Quality-Adapted Carotenoid Photoprotection in the Photosystem of Roseiflexus castenholzii. J. Phys. Chem. Lett.  15 (12): 3470-3477.

Zhao S, Shen L, Li X, Tao Q, Li Z, Xu C, Zhou C, Yang Y, Sang M, Han G, Yu LJ, Kuang T, Shen JR, Wang W (2023). Structural insights into photosystem II supercomplex and trimeric FCP antennae of a centric diatom Cyclotella meneghiniana. Nat Commun. 14 (1): 8164.

Tani K, Kanno R, Ji XC, Satoh I, Kobayashi Y, Hall M, Yu LJ, Kimura Y, Mizoguchi A, Humbel BM, Madigan MT, Wang-Otomo ZY (2023). Rhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex. Nat Commun. 14 (1): 846.

Hao JF, Yamano N, Qi CH, Zhang Y, Ma F, Wang P, Yu LJ, Zhang JP (2023). Carotenoid-Mediated Long-Range Energy Transfer in the Light Harvesting-Reaction Center Complex from Photosynthetic Bacterium Roseiflexus castenholzii. J Phys Chem B. 127 (48): 10360-10369.

Qi CH#, Wang GL#, Wang FF#, Xin Y, Zou MJ, Madigan MT, Wang-Otomo ZY, Ma F*, and Yu LJ* (2023). New insights on the photocomplex of Roseiflexus castenholzii revealed from comparisons of native and carotenoid-depleted complexes. J. Biol. Chem. 299, 105057. 

Satoh I, Gotou K, Nagatsuma S, Nagashima KVP, Kobayashi M, Yu LJ, Madigan MT, Kimura Y, and Wang-Otomo ZY (2023). Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria. Biochim Biophys Acta Bioenerg 1864, 149001. 

Liu XH, Liu PC, Li HJ, Xu Z, Jia L, Xia Y, Yu ML, Tang WQ, Zhu XL, Chen C, Zhang Y, Nango EK, Tanaka R, Luo FJ, Kato K, Nakajima Y, Kishi S, Yu HX, Matsubara N, Owada S, Tono K, Iwata S, Yu LJ*, Shen JR*, Wang JY* (2022). Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser. Nat Chem. 14: 1054-1060. 

Ma F#, Romero E, Jones MR, Novoderezhkin VI, Yu LJ#, van Grondelle R (2022). Dynamics of diverse coherences in primary charge separation of bacterial reaction center at 77 K revealed by wavelet analysis. Photosynth. Res. 151: 225-234. 

Zhang Y, Qi CH, Yamano N, Wang P, Yu LJ*, Wang-Otomo ZY, Zhang JP* (2022). Carotenoid Single-Molecular Singlet Fission and the Photoprotection of a Bacteriochlorophyll b-Type Core Light-Harvesting Antenna. J Phys Chem Lett. 13 (16):3534-3541. 

Tani K, Kanno R, Kurosawa K, Takaichi S, Nagashima KVP, Hall M, Yu LJ, Kimura Y, Madigan MT, Mizoguchi A, Humbel BM, Wang-Otomo Z-Y (2022). An LH1–RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins. Commun Biol. 5 (1): 1197.  

Tani K, Kobayashi K, Hosogi N, Ji X C, Nagashima S, Nagashima KVP, Izumida A, Inoue K, Tsukatani Y, Kanno R, Hall M, Yu LJ, Ishikawa I, Okura Y, Madigan MT, Mizoguchi A, Humbel BM, Kimura Y, Wang-Otomo ZY (2022). A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes. J Biol Chem. 298 (6), 101967. 

Kimura Y, Nojima S, Nakata K, Yamashita T, Wang XP, Takenaka S, Akimoto S, Kobayashi M, Madigan MT, Wang-Otomo ZY#, and Yu LJ.# (2021). Electrostatic charge controls the lowest LH1 Q(y) transition energy in the triply extremophilic purple phototrophic bacterium, Halorhodospira halochloris. Biochim. Biophys. Acta Bioenerg. 1862: 148473. (#co-respondent) 

Zheng DD*, Tao M*, Yu LJ*, Liu XH, Xia AD, Wang JY. (2021) Ultrafast Photoinduced Electron Transfer in a Photosensitizer Protein. CCS Chemistry. 3: 1580–1586. (*equal contribution) 

Yang ZH* Qi CH*, Liu WZ, Yin DB, Yu LJ#, Li LD#, Guo XF.# (2021) Revealing conformational transition dynamics of photosynthetic proteins in single-molecule electrical circuits. J. Phys. Chem. Lett. 12: 3853-3859. (*equal contribution, #co-respondent) 

Wang J*, Yu LJ*, Wang WD, Yan, QJ, Kuang TY, Qin XC, Shen J R. (2021) Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 A resolution. J. Integr. Plant Biol. 63: 1367-1381. (*equal contribution) 

Ma F#, Romero E, Jones MR, Novoderezhkin VI, Yu LJ#, van Grondelle R. (2021) Dynamic stark effect in two-dimensional spectroscopy revealing modulation of ultrafast charge separation in bacterial reaction centers by an inherent electric field. J. Phys. Chem. Lett. 12: 5526-5533. (#co-respondent) 

Kimura Y, Yamashita T, Seto R, Imanishi M, Honda M, Nakagawa S, Saga Y, Takenaka S, Yu LJ, Madigan MT, Wang-Otomo ZY. (2021) Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes. Photosynth. Res. 148: 77-86. 

Kawakami T*, Yu LJ*#, Liang T, Okazaki K, Madigan MT, Kimura Y, Wang-Otomo Z Y#. (2021). Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP. Nat. Commun. 12: 1104. (*equal contribution, # co-respondent). 

Yu LJ, Ma F. (2020) Recent Progress on the LH1-RC Complexes of Purple Photosynthetic Bacteria. In Microbial Photosynthesis, Springer: pp 53-72. 

Tani K, Kanno R, Makino Y, Hall M, Takenouchi M, Imanishi M, Yu LJ, Overmann J,  Madigan MT, Kimura Y, Mizoguchi A, Humbel BM, Wang-Otomo ZY. (2020) Cryo-EM structure of a Ca(2+)-bound photosynthetic LH1-RC complex containing multiple alphabeta-polypeptides. Nat. Commun. 11: 4955. 

Zou MJ, Mu Y, Chai X, Ouyang M, Yu LJ, Zhang LX, Meurer J, Chi W. (2020) The critical function of the plastid rRNA methyltransferase, CMAL, in ribosome biogenesis and plant development. Nucleic Acids Res. 48: 3195-3210. 

Wang W*, Yu LJ*, Xu C, Tomizaki T, Zhao S, Umena Y, Chen X, Qin X, Xin Y, Suga M, Han G, Kuang T,  Shen JR. (2019) Structural basis for blue-green light harvesting and energy dissipation in diatoms. Science. 363: eaav0365. (*equal contribution) 

Yu LJ, Suga M, Wang-Otomo ZY, Shen JR. (2018). Structure of photosynthetic LH1-RC supercomplex at 1.9 Å resolution. Nature. 556: 209-213. 

Yu LJ, Suga M, Wang-Otomo ZY, Shen JR. (2018) Novel features of LH1-RC from Thermochromatium tepidum revealed from its atomic resolution structure. FEBS J. 285: 4359-4366. 

Chen JH*, Yu LJ*, Boussac A, Wang-Otomo ZY, Kuang T, Shen JR. (2018) Properties and structure of a low-potential, penta-heme cytochrome c552 from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum. Photosynth. Res. 139: 281-293. (*equal contribution) 

Kimura, Y.; Kawakami, T.; Arikawa, T.; Li, Y., Yu, L. J.; Ohno, T.; Madigan, M. T.; Wang-Otomo, Z. Y.(2018). C-terminal cleavage of the LH1 alpha-polypeptide in the Sr2+-cultured Thermochromatium tepidum. Photosynth. Res. 135: 23-31. 

Yu, J.; Fu, L. M.; Yu, L. J.; Shi, Y.; Wang, P.; Wang-Otomo, Z. Y.; Zhang, J. P.(2017). Carotenoid singlet fission reactions in bacterial light harvesting complexes as revealed by triplet excitation profiles. J. Am. Chem. Soc. 139: 15984-15993. 

Suga M, Akita F, Sugahara M, Kubo M, Nakajima Y, Nakane T, Yamashita K, Umena Y, Nakabayashi M, Yamane T, Nakano T, Suzuki M, Masuda T, Inoue S, Kimura T, Nomura T, Yonekura S, Yu LJ, Sakamoto T, Motomura T, Chen JH, Kato Y, Noguchi T, Tono K, Joti Y, Kameshima T, Hatsui T, Nango E, Tanaka R, Naitow H, Matsuura Y, Yamashita A, Yamamoto M, Nureki O, Yabashi M, Ishikawa T, Iwata S, Shen JR. (2017). Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL. Nature. 543: 131-135. 

Nagashima KVP, Sasaki M, Hashimoto K, Takaichi S, Nagashima S, Yu LJ, Abe Y, Gotou K, Kawakami T, Takenouchi M, Shibuya Y, Yamaguchi A, Ohno T, Shen JR, Inoue K, Madigan MT, Kimura Y, Wang-Otomo ZY. (2017). Probing structure–function relationships in early events in photosynthesis using a chimeric photocomplex. Proc. Natl. Acad. Sci. 114: 10906-10911. 

Ma F, Yu LJ, Llansola-Portoles MJ, Robert B, Wang-Otomo ZY, van Grondelle R. (2017). Metal cations induced alphabeta-bchl a heterogeneity in lh1 as revealed by temperature-dependent fluorescence splitting. Chemphyschem. 18: 2295-2301. 

Ma F, Yu LJ, Hendrikx R, Wang-Otomo ZY, van Grondelle R. (2017) Excitonic and vibrational coherence in the excitation relaxation process of two lh1 complexes as revealed by two-dimensional electronic spectroscopy. J. Phys. Chem. Lett. 8: 2751-2756. 

Ma F, Yu LJ, Hendrikx R, Wang-Otomo ZY, van Grondelle R. (2017) Direct observation of energy detrapping in lh1-rc complex by two-dimensional electronic spectroscopy. J. Am. Chem. Soc. 139: 591-594. 

Yu LJ, Kawakami T, Kimura Y, Wang-Otomo ZY. (2016) Structural basis for the unusual qy red-shift and enhanced thermostability of the lh1 complex from Thermochromatium tepidum. Biochemistry. 55: 6495-6504. 

Ma F, Yu LJ, Ma XH, Wang P, Wang-Otomo ZY, Zhang JP. (2016) Bacterial light-harvesting complexes showing giant second-order nonlinear optical response as revealed by hyper-rayleigh light scattering. J. Phys. Chem. B. 120: 9395-401. 

Kimura Y, Yura Y, Hayashi Y, Li Y, Onoda M, Yu LJ, Wang-Otomo ZY, Ohno T. (2016) Spectroscopic and thermodynamic characterization of the metal-binding sites in the lh1-rc complex from thermophilic photosynthetic bacterium Thermochromatium tepidum. J. Phys. Chem. B. 120: 12466-12473. 

Huo YL, Shi Y, Wang QY, Li L, Yu LJ, Wang P, Zhang JP, Wang, ZY (2016) Spectroscopic properties of LH2 from Thermochromatium tepidum in liposome and detergent micelles. Chem. J. Chin. Univ. 37: 1678-1685. 

Ma F, Yu LJ, Wang-Otomo ZY, van Grondelle R (2016) Temperature dependent LH1-->RC energy transfer in purple bacteria Tch. tepidum with shiftable LH1-Q band: A natural system to investigate thermally activated energy transfer in photosynthesis. Biochim. Biophys. Acta. 1857: 408-414. 

Shi Y, Zhao NJ, Wang P, Fu LM, Yu LJ, Zhang JP, Wang-Otomo ZY. (2015) Thermal adaptability of the light-harvesting complex 2 from Thermochromatium tepidum: Temperature-dependent excitation transfer dynamics. J. Phys. Chem. B. 119: 14871-14879. 

Ma F, Yu LJ, Wang-Otomo ZY, van Grondelle R.. (2015) The origin of the unusual Qy red shift in LH1-RC complexes from purple bacteria Thermochromatium tepidum as revealed by Stark absorption spectroscopy. Biochim. Biophys. Acta. 1847: 1479-1486. 

Kimura Y, Kawakami T, Yu LJ, Yoshimura M, Kobayashi M, Wang-Otomo ZY. (2015) Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. FEBS Lett. 589: 1761-5. 

Niwa S*, Yu LJ*, Takeda K, Hirano Y, Kawakami T, Wang-Otomo ZY, Miki K. (2014) Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0Å. Nature. 508: 228-32. (*equal contribution) 

Yu LJ, Unno M, Kimura Y, Yanagimoto K, Oh-oka H, Wang-Otomo ZY. (2013) Structure analysis and characterization of the cytochrome c-554 from thermophilic green sulfur photosynthetic bacterium Chlorobaculum tepidum. Photosynth. Res. 118: 249-258. 

Kimura Y, Inada Y, Yu L J, Ohno T, Wang Z. (2013) Strontium ions are functionally replaceable with calcium ions in the light-harvesting 1 reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. In Photosynthesis Research for Food, Fuel and the Future. (eds. Kuang, T. Y. et al.). 105-109 (Springer). 

Sekine F, Horiguchi K, Kashino Y, Shimizu Y, Yu LJ, Kobayashi M, Wang ZY. (2012) Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum. Photosynth. Res. 111: 9-18.

Yang F, Yu LJ, Wang P, Ai XC, Wang ZY, Zhang JP. (2011) Effects of Aggregation on the excitation dynamics of LH2 from Thermochromatium tepidum in aqueous phase and in chromatophores. J. Phys. Chem. B. 115: 7906-7913. 

Kimura Y, Inada Y, Yu LJ, Wang ZY, Ohno T.  (2011) A Spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum. Biochemistry. 50: 3638-3648. 

Yu LJ, Kato S, Wang ZY. (2010) Examination of the putative Ca2+-binding site in the light-harvesting complex 1 of thermophilic purple sulfur bacterium Thermochromatium tepidum. Photosynth. Res. 106: 215-220. 

Yang F, Yu LJ, Wang P, Ai XC, Wang ZY, Zhang JP. (2010) Excitation Dynamics of the Light-Harvesting complex 2 from Thermochromatium tepidum. Acta. Phys-Chim. Sin. 26: 2021-2030. 

Kimura Y, Yu LJ, Hirano Y, Suzuki H, Wang ZY. (2009) Calcium Ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. J. Biol. Chem. 284: 93-99. 

Ma F, Kimura Y, Yu LJ, Wang P, Ai XC, Wang ZY, Zhang JP. (2009) Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling. FEBS J. 276: 1739-1749. 

Kimura Y, Hirano Y, Yu LJ, Suzuki H, Kobayashi M, Wang ZY. (2008) Calcium ions are involved in the unusual red shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum. J. Biol. Chem. 283: 13867-13873.